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Anti-Nuclear Antibody ELISA Screen Reflex IFA

Mnemonic

ANA R-IFA

Synonym

ANA - ANA ELISA - Antinuclear Antibody - ANA IgG Screen - ANA Screen - antinuclear Ab

Includes

Anti-Nuclear Antibody (ANA) ELISA Screen with Reflex to IFA Titer and Pattern

Guidelines

ANA specimens are screened using an ELISA assay. All specimens that do not screen negative are confirmed using HEp-2 cells, and if positive, the titer and pattern will be reported.

Specimen Collection Requirements

Collect

Serum, one 5.0 mL gold (SST) top tube

Supply Item Number - 101235

Handling

Allow blood to clot for 30 minutes. Centrifuge and separate serum from cells immediately. Pour serum into plastic tube.

Preferred Volume

1.0 mL serum

Minimum Volume

0.5 mL serum (whole blood 1.2 mL minimum draw)

Transport

Refrigerated if arrival in the Legacy Central Laboratory is within 48 hours. Freeze if transport requires longer than 48 hours.

Rejection Criteria

Plasma and other body fluids; severely lipemic or bacterially contaminated specimens

Stability

COLLECTION TO INITIATION OF TESTING AFTER SEPARATION FROM CELLS: Ambient: 8 hours; Refrigerated (2-8°C): 48 hours; Frozen (at or below -20°C): 2 months

Testing Information

Performed

Mon - Fri

Reported

24-72 hours (ANA Screen)

Method

Enzyme-Linked Immunosorbent Assay (ELISA); Indirect Fluorescent Antibody

Reference Values

Reference Values

Negative (ELISA)

CPT Codes

86038 ANA; if reflexed, add 86039 ANA titer

Last Updated: Wednesday, January 27, 2021 12:41:07 AM


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