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Antibody Identification, Red Cell

Mnemonic

BB ABID

Synonym

Antibody Identification - Antibody Identification Panel - Red Cell Panel - Elution Technique

Includes

Antibody identification; Direct Antiglobulin Test (DAT), Prewarm technique, Antibody elution and Antigen typing will be performed if indicated. Antibodies identified during prenatal testing and known to cause hemolytic disease of the newborn will be titered.

Guidelines

Antibody identification is performed using the plasma of patients who have a positive indirect antiglobulin test (Antibody Screen) to identify clinically significant antibodies. Also necessary before transfusion if antibody screen is positive. Used to work up obstetric patient's positive antibody screen.

Specimen Collection Requirements

Collect

Three full 4.0 mL lavender (EDTA) top tubes

Handling

Tubes must be labeled appropriately. See Blood Bank Specimen Collection Guidelines.

Preferred Volume

Three full 4.0 mL lavender (EDTA) top tubes

Minimum Volume

Two full 4.0 mL lavender (EDTA) top tubes

Transport

Ambient or refrigerated

Rejection Criteria

Gross hemolysis, incorrect specimen container, incorrectly labeled or frozen specimen

Stability

Ambient: 24 hours; Refrigerated: 3 days; Frozen: Unacceptable

Testing Information

Performed

7 days/week

Reported

2 hours - 2 days depending on complexity

Method

Panel of separate, selected RBC's are exposed to the patient's plasma or to eluate

Reference Values

Comments

The presence of antibodies can limit the number of compatible units available. When an antibody is identified, it is necessary to confirm that the patient's cells and the donor unit cells lack the corresponding antigens. Full crossmatch is performed following identification of clinically significant antibodies.

Limitations:  Antibody may be too weak to be detected and/or identified. Antibodies to low incidence antigens may not be detected. Presence of auto-antibodies significantly complicates identification of alloantibodies and may in some cases mask their presence.

CPT Codes

86870

Last Updated: Thursday, October 17, 2019 09:18:04 PM


Client Services: 503-413-1234