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Antibody Screen

Mnemonic

BB ABSC - Gel (inpatient); ABSC-OTR (outpatient)

Synonym

Indirect Antiglobulin Test

Includes

This is a screen for unexpected red blood cell antibodies which may be found in patient plasma.

Guidelines

Used to screen for unexpected red blood cell antibodies in plasma by use of red cells with known antigenic makeup. Useful in pre-transfusion testing, antibody identification, prenatal screening and evaluation of potential causes of hemolysis. A positive test must be followed up with antibody identification.

Specimen Collection Requirements

Collect

Adult: Two full 4.0 mL lavender (EDTA) top tubes; Neonates (<4 months): Two full lavender top microtainer tubes; Pediatrics (≥4 months): 3-6 full lavender top microtainer tubes

Supply Item Number - 158386

Handling

Tubes must be labeled appropriately. See Blood Bank Specimen Collection Guidelines.

Preferred Volume

Two full 4.0 mL lavender (EDTA) top tubes

Minimum Volume

Two lavender (EDTA) top microtainer tubes (Whole Blood 1.0 minimum draw; Neonates <4 months: one lavender (EDTA) top microtainer tube (Whole Blood 0.5 mL minimum draw)

Transport

Ambient or refrigerated

Rejection Criteria

Gross hemolysis, incorrect specimen container, incorrectly labeled or frozen specimen

Stability

Ambient: 24 hours; Refrigerated: 3 days; Frozen: Unacceptable

Testing Information

Performed

7 days/week

Reported

2-24 hours, for routine testing

Method

Gel testing with anti-IgG

Reference Values

Reference Values

Negative

Comments

Abnormal proteins and cold auto agglutinins may interfere and cause delays in interpretation. Low titer antibodies and antibodies to low incidence antigens may not be detected.

CPT Codes

86850

Last Updated: Thursday, October 17, 2019 08:54:25 PM


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