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Antibody Titer

Mnemonic

BB ABTITER

Synonym

Rh Titer

Includes

Determination of antibody titer by serial dilution.

Guidelines

Measures titer in antenatal maternal serum when antibody screen is positive. Useful to follow the development of antibody formation in cases of maternal-fetal blood incompatibility.

Specimen Collection Requirements

Collect

Three full 4.0 mL lavender (EDTA) top tubes

Handling

Tubes must be labeled appropriately. See Blood Bank Specimen Collection Guidelines.

Preferred Volume

Three full 4.0 mL lavender (EDTA) top tubes

Minimum Volume

Two full 4.0 mL lavender (EDTA) top tubes

Transport

Ambient or refrigerated

Rejection Criteria

Gross hemolysis, incorrect specimen container, incorrectly labeled or frozen specimen

Stability

Ambient: 24 hours; Refrigerated: 3 days; Frozen: Unacceptable

Testing Information

Note

Remaining plasma is frozen for subsequent parallel titers.

Performed

Mon - Fri 7:00 am - 3:30 pm

Reported

2 hours - 4 days, depending on complexity and time of specimen arrival

Method

Serial dilution, saline incubation followed by Antihuman globulin technique (AHG, Coombs')

Reference Values

Reference Values

An Anti-D titer of 16 is considered significant.
Critical titers for antibodies other than Anti-D have not been well defined.
A three-fold rise in titer is considered significant.

Comments

Antibody detection and identification must be completed prior to performing a titer. If a clinically significant antibody is present, then it will be titered in antenatal work-up. A rising titer of an antibody known to cause hemolytic disease of the newborn is more significant than a single assay. Phenotypic determination of the father's RBCs can be helpful.

Limitations: While correlation does exist between titer and severity of erythroblastosis, titers do not always reflect the condition of an unborn child. False-negatives and false-positives occur. Abnormal results may indicate the need for amniocentesis.

CPT Codes

86886

Last Updated: Thursday, October 17, 2019 09:19:24 PM


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