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* B-Type Natriuretic Peptide

Mnemonic

BNP

Synonym

BNP

Specimen Collection Requirements

Collect

Plasma, one 4.0 mL lavender (EDTA) top plastic tube

View image for Supply Item Number - 158386

Outreach clients: Due to short stability and processing requirements for this test, sharing BNP specimen with other testing is not recommended. Please submit one specimen for B-Type Natriuretic Peptide and a second EDTA specimen for other requests.

If two specimens are not submitted, we may need to cancel the BNP test due to specimen stability.

Handling

Centrifuge and separate EDTA plasma from cells within 7 hours of collection. Freeze plasma if testing cannot be performed within 24 hours of collection. 

 

Note: Freezing EDTA plasma within 24 hours of collection is critical to preserve integrity of specimen for testing due to preanalytical process for this test.

 

Before testing, allow samples to thaw at room temperature for 30 minutes before testing. Mix well. Thaw samples only once.

Preferred Volume

1.0 mL EDTA Plasma

Minimum Volume

0.5 mL EDTA Plasma (1.2 mL minimum whole blood draw)

Transport

EDTA WHOLE BLOOD: Refrigerated (2-8°C). Should be received no later than 4 hours from collection to allow for timely processing.

 

EDTA PLASMA: Refrigerated (2-8°C). If transport is delayed longer than 24 hours from collection, plasma should be frozen (≤ -20°C).

Rejection Criteria

  • Clotted specimens
  • Grossly hemolyzed specimens
  • Specimens collected in tubes other than EDTA plastic tubes
  • Whole blood specimens greater than 7 hours old
  • Specimens that have been thawed more than once

Stability

EDTA WHOLE BLOOD: Room Temperature (18-26°C): 7 hours; Refrigerated (2-8°C): 7 hours; Frozen (≤ -20°C): Unacceptable

EDTA PLASMA: Room Temperature (18-26°C): 7 hours; Refrigerated (2-8°C): 24 hours; Frozen (≤ -20°C): 1 month  (NOTE: STABILITY IS FROM TIME OF COLLECTION AND NOT CENTRIFUGATION.)

Do not thaw more than once

Testing Information

Performed

7 days/week

Reported

24 hours

Method

Chemiluminescent Immunoassay

Reference Values

Reference Values

All: 5-99 pg/mL

CPT Codes

83880

Last Updated: Wednesday, February 10, 2021 12:08:25 AM


Client Services: 503-413-1234