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Bilirubin, Total, Body Fluids

Mnemonic

BF BIL T

Synonym

BF BIL T - BF TBILI

Guidelines

Do not order this test for amniotic fluid, see Bilirubin, Amniotic Fluid

For a list of acceptable body fluids for this test, refer to Chemistry Testing Performed on Cerebral Spinal Fluid and Other Body Fluids  

Specimen Collection Requirements

Collect

Body fluid, one sterile container. Do not use tubes with anti-coagulant. Indicate source of body fluid on the collection container and test request form. If more than one tube is collected, each tube should be labeled with a number indicating the sequence in which it was collected.

Handling

Protect specimen from light by wrapping container with foil or other light-opaque material. Specimen may be transferred to an amber screw-cap transport tube as an alternate method of light protection. If cellular material is present, centrifuge specimen and remove supernatant within four hours of collection.

Preferred Volume

1.0 mL

Minimum Volume

0.25 mL

Transport

Refrigerated (2-8°C) and protected from light.

Rejection Criteria

Gastric fluids, fluids collected with anti-coagulant, fluids from sources outside the body, and intraosseous specimens will not be accepted.

Stability

PROTECTED FROM LIGHT:  Room Temperature (18-26°C): 24 hours, Refrigerated (2-8°C): 3 days, Frozen (≤ -20°C): 1 month

Testing Information

Note

Bilirubin is photosensitive, failure to protect the specimen from light will cause a decrease in measured result.

Performed

7 days/week

Reported

24 hours

Method

Colorimetry

Reference Values

Reference Values

Not established

Comments

Metabolites of acetaminophen (N-acetyl-p-benzoquinone imine) will generate erroneously low results in samples of patients that have taken toxic doses of acetaminophen.

 

Eltrombopag and its metabolites may interfere with this assay causing erroneously high patient results.

CPT Codes

82247

Last Updated: Monday, January 25, 2021 10:52:05 PM


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