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* Calcium, Ionized, Serum (Outreach)

Mnemonic

CA ION S

Synonym

Ionized Calcium Outreach Serum - Calcium Ionized Serum Level - Calcium Free - Calcium - iCa

Guidelines

To be used for all Outreach and Outpatient testing only.

Specimen Collection Requirements

Collect

Serum, one 5.0 mL gold (SST) top tube

Due to processing requirements for this test, sharing specimen with other testing is not feasible. If other tests are ordered, please submit separate specimen(s).

Handling

All specimens should be collected under anaerobic conditions. For anaerobic samples, do not remove the top of the tube before analysis. Any loss of CO2 will increase the pH, which decreases ionized calcium. Do not store specimens on ice. Allow blood in gold top tube to clot completely, at least 30 minutes. Spin tube within 1 hour of collection. Do not remove top from tube.

NOTE: If testing of serum will be delayed by more than 72 hours, the sample may be aliquoted and frozen. Exposure to air must be minimized as much as possible during the aliquoting process.

Preferred Volume

1.0 mL Serum

Minimum Volume

0.5 mL (1.2 mL minimum whole blood draw)

Transport

Refrigerated (2-8°C)

Rejection Criteria

Severely hemolyzed specimens. Specimens collected using one of these

anticoagulants:  Lithium heparin in mint green top tube, sodium heparin,

EDTA, citrate, and potassium oxalate/sodium fluoride

Stability

UNSPUN SAMPLES: Room Temperature (18-26°C): 1 hour

AFTER SEPARATION FROM CELLS: Room Temperature (18-26°C): 8 hours; Refrigerated (2-8°C): 3 days; Frozen (≤-20°C): 4 days

 

If samples are frozen, thaw only once before testing.

Testing Information

Performed

7 days/week

Reported

24 hours

Method

Ion Selective Electrode

Reference Values

Reference Values

Critical Values

CPT Codes

82330

Last Updated: Wednesday, January 13, 2021 01:32:38 AM


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