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Culture, Stool Pathogens

Mnemonic

C ST

Synonym

Stool Culture

Includes

Culture includes testing for Salmonella, Shigella, Campylobacter, E. coli 0157, Aeromonas sp. and Yersinia. Vibrio on request. Culture also includes Shiga-toxin testing on all stools per CDC recommendations.

Guidelines

See also Gastrointestinal Panel By PCR

Submit specimen during the acute stage of infection. Do NOT submit specimens from patients on broad spectrum antibiotics or after the third hospitalization day, consider C. difficile testing instead. A single stool specimen has a sensitivity of >95% for detection of enteric bacterial pathogens causing enterocolitis. If the initial sample is negative, then one additional specimen may be submitted.

Specimen Collection Requirements

Collect

Stool collected in a clean container and placed in Enteric Transport (Cary Blair) within one hour of collection

Patient Instructions for Stool Culture and Gastrointestinal Panel: English - Spanish - Russian - Vietnamese

View Stool Collection Guide

Handling

Preserve stool in Enteric Transport provided by Legacy Laboratory Services within one hour of collection. See Specimen Collection-Transport Guide for Stool Orders. Fill the enteric transport vial until liquid reaches the line on the label, approximately 0.5 gm of stool.
Note: If a minimal amount of stool is received, it is acceptable to discard some of the Enteric Transport before adding stool. Keep approximately a 1:4 ratio of stool to Enteric Transport.

Preferred Volume

5.0 gm

Minimum Volume

0.5 gm

Transport

Stool in Enteric Transport at 20-25°C

Rejection Criteria

Leaky or dry specimen, delayed transport more than 1 hour without use of Enteric Transport and specimens collected from bedpans, contaminated with urine, frozen specimen

Stability

Ambient: 1 hour; In Enteric Transport: 72 hours, ambient or refrigerated

Testing Information

Note

Specify if Vibrio is to be ruled out. Selective medium for recovery of Vibrio is not included in routine set-up.

Performed

7 days/week

Reported

72 hours

Method

Culture

Reference Values

Reference Values

Negative for Salmonella, Shigella, Campylobacter, E. coli 0157, Yersinia, Aeromonas, and Shigatoxins.

Comments

Positive samples will be sent and/or reported to Oregon State Public Health as required by State law.
Any Shiga-toxin positive stool that has no E. coli 0157 isolated will be sent to the Oregon State Public Health Lab for typing of the pathogen. Additional charges for Vibrio culture (CPT 87046), if ordered.

 

Stool Culture includes culture to rule-out Salmonella, Shigella, (CPT 87045), Campylobacter, Yersinia, and E. coli 0157 (CPT 87046 each plate) and an EIA for Shiga-like toxin from E. coli (CPT 87427). 

 

Additional charges as needed for identification and to differentiate normal flora from pathogens (CPT 87077). Susceptibility tests are usually not performed unless specifically requested.  A susceptibility will always be performed if Shigella is isolated (Possible CPT 87186, 87184, 87181).

CPT Codes

87045, 87046 (X3), 87427 (87077, 87186, 87184, 87181 as needed)

Last Updated: Tuesday, January 12, 2021 01:57:49 AM


Client Services: 503-413-1234