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HLA-B27

Mnemonic

HLA_B27

Synonym

HLAB27 TYP - HLAB27 – HLA_B27 - HLA-B27 - HLA B27, HLA-B27 Typing - HLA-B27 TYPE

Guidelines

May assist in the diagnosis of ankylosing spondylitis and a few other rheumatic disorders. HLA-B27 testing is routinely used to screen for AS since 90% of patients with AS have the HLA-B27 surface antigen compared to only 8% of healthy individuals.

HLA-B27 is a serologically defined allele of the human HLA-B locus. The presence of the HLA-B27 antigen is strongly associated with ankylosing spondylitis and related disorders. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions. Anti-HLA-B27 may cross-react with several of the HLA-B antigens, most commonly with the HLA-B7 antigen, leading to a low level of false-positive results. Clinical correlation is required for complete interpretation.

Specimen Collection Requirements

Collect

Whole Blood, one 4.0 mL lavender (EDTA) or 4.0 mL green (sodium heparin) top tube; minimum ½ full

Handling

Store Ambient (20°-25°C); refrigerated or frozen specimens unacceptable. The specimen must be analyzed within 96 hours of collection.

Preferred Volume

4.0 mL lavender (EDTA) OR green top (sodium heparin) top tube must contain between 2.0 - 4.0 mL whole blood.

2.0 mL lavender (EDTA) OR green top (sodium heparin) top tube must contain between 1.0 - 2.0 mL whole blood.

Minimum Volume

Whole Blood, lavender (EDTA) OR green (sodium heparin) top tube must be filled to a minimum of 1/2 of the maximum sample volume

  • 4.0 mL tube must have 2.0 mL of blood minimum
  • 2.0 mL tube must have 1.0 mL of blood minimum
  • Micro lavender (EDTA) top container must be filled between the minimum and maximum fill lines

Transport

Ambient (20°-25°C) 

Rejection Criteria

Refrigerated, frozen, clotted or hemolyzed specimens, Yellow (ACD) top tube, and specimens > 96 hours (4 days) old.

Stability

Ambient (20°-25°C): 96 hours 

Testing Information

Performed

Monday - Friday except holidays

Reported

Monday -Friday except holidays, 24-48 hours from receipt in the Flow Lab

Method

Flow Cytometry

Reference Values

Reference Values

Negative

CPT Codes

86812

Last Updated: Thursday, March 14, 2019 12:50:48 PM


Client Services: 503-413-1234