Insulin, Fasting Mnemonic INSUL FAST Specimen Collection Requirements Patient Preparation Patient must fast for 8-14 hours before blood is drawn. Collect Serum, one 5.0 mL gold (SST) or 7.0 mL red top tube Supply Item Number - 101235 or 101192 Handling Allow serum to clot completely at room temperature (minimum -SST: 30 minutes, red top tubes: 60 minutes). Centrifuge and separate serum from cells ASAP. Preferred Volume 1.0 mL Serum Minimum Volume 0.5 mL Serum (1.2 mL minimum whole blood draw) Transport Frozen (≤ -20°C) Rejection Criteria Grossly hemolyzed specimens Plasma specimens Specimens frozen and thawed more than once Stability AFTER SEPARATION FROM CELLS: Room Temperature (18-26°C): 8 hours; Refrigerated (2-8°C): 24 hours; Frozen (≤ -20°C): 2 months. Do NOT freeze/thaw specimens more than once. Testing Information Performed Mon, Wed, Fri Reported 24-72 hours Method Chemiluminescent Immunoassay Reference Values Reference Values 1.9 - 23.0 µIU/mL CPT Codes 83525 Last Updated: Thursday, February 11, 2021 07:46:35 PM Client Services: 503-413-1234