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Iron Deficiency Profile

Please see LabAlert: New Reference Ranges for UIBC and TIBC.

Mnemonic

IRON DEF

Synonym

TOTAL IRON BINDING CAPACITY - TIBC - IRON SATURATION - TRANSFERRIN SATURATION - FERRITIN - FED

Includes

Ferritin, Iron, UIBC, Total Iron Binding Capacity (TIBC), Iron Saturation (Transferrin Saturation)

Specimen Collection Requirements

Patient Preparation

Specimen should be collected in the morning from patients who have fasted for at least 8 hours. Iron levels decrease by 30% during the course of the day. The patient should not take iron supplements at least 24 hours before blood is drawn.

Collect

Serum or Plasma, one 5.0 mL gold (SST), 7.0 mL red, or 3.0 mL mint green (lithium heparin, PST) top tube

Handling

Allow serum to clot completely at room temperature (minimum: 30 minutes). Centrifuge and separate serum from cells ASAP. Avoid contamination with environmental iron. Use only Iron-free collection tubes, pipets and test tubes.

Preferred Volume

1.0 mL Serum/Plasma

Minimum Volume

0.5 mL Serum/Plasma (1.2 mL minimum whole blood draw)

Transport

Refrigerated (2-8°C)

Rejection Criteria

Moderately hemolyzed specimens

Stability

SERUM OR PLASMA (SEPARATED FROM CELLS, POST COLLECTION): Room Temperature (18-26°C): 8 hours; Refrigerated (2-8°C): 2 days; Frozen (≤-20°C): 1 month

Testing Information

Note

Iron is performed to calculate TIBC and Iron Saturation.

Performed

7 days/week

Reported

24 hours

Method

Colorimetry, Chemiluminescent Immunoassay

Reference Values

Reference Values

CPT Codes

Iron: 83540; IBC: 83550; Ferritin: 82728

Last Updated: Wednesday, February 3, 2021 01:27:20 AM


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