JAK2 GENE Mnemonic JAK2 GENE Synonym JAK2 - JAK2 RGQ PCR - V617F mutation testing Includes JAK2 p. Val617Phe (V617F) mutation, Qualitative (Does not include exon 12) Specimen Collection Requirements Collect Whole Blood, one 4.0 mL lavender (EDTA) top tube or Bone Marrow, one 3.0 mL lavender (EDTA) top tube Handling Whole blood sample must be submitted in original container. Aliquot tubes will not be accepted. Do not separate whole blood. Do not freeze Preferred Volume Whole Blood, 4.0 mL or Bone Marrow, 3.0 mL Minimum Volume Whole Blood, 1.0 mL or Bone Marrow, 0.5 mL Transport Ambient Temperature Rejection Criteria Frozen or clotted samples, serum or severly hemolyzed whole blood, specimens collected in anticoagulants other than EDTA Stability Ambient: 10 days; Refrigerated: 10 days; Frozen: Unacceptable Testing Information Note Critical sterile. Samples cannot be shared with other laboratory departments unless molecular testing is performed first. Performed Weekly Reported 4-10 days Method Polymerase Chain Reaction Reference Values Reference Values Not Detected Comments Isolated DNA can be sent for referral testing (i.e CALR). CPT Codes 81270 Last Updated: Thursday, March 14, 2019 12:50:51 PM Client Services: 503-413-1234