LAMELLAR BODY COUNT, AMNIOTIC FLUID Mnemonic AF LBC Synonym LBC - Lamellar Body - Lamellar Bodies - FLM - Fetal Lung Maturity Specimen Collection Requirements Collect Amniotic fluid, one sterile container, collected via trans-abdominal amniocentesis Handling Do not centrifuge or filter specimens. Keep the specimen refrigerated (2-8°C). Do not freeze specimen. Do not let specimens sit directly on cold packs. Insulate specimen with bubble wrap or other appropriate packing material when shipped cold. Preferred Volume 2.0 mL Minimum Volume 0.75 mL Transport Refrigerated (2-8°C) Rejection Criteria Frozen, centrifuged, filtered, visible blood or hemolysis, contaminated with meconium, vaginal pool or highly viscous specimens Stability Ambient (20-25°C): 7 days; Refrigerated (2-8°C): 28 days; Frozen: Unacceptable Testing Information Performed 7 days/week Reported 24 hours once received at reference laboratory Method Automated Cell Counter - Impedance Method Referral Lab Mayo (LBC) Reference Values Reference Values Immature: <15,000/mcL Indeterminate: 15,000-50,000/mcL Mature: >50,000/mcL CPT Codes 83664 Last Updated: Thursday, March 14, 2019 12:50:52 PM Client Services: 503-413-1234