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Methadone, Quantitative, Serum or Plasma

Mnemonic

METHAD

Synonym

Dolophine - Methadose

Specimen Collection Requirements

Collect

Serum or Plasma, one 7.0 mL red, 4.0 mL dark green (sodium heparin) or 4.0 mL Lavender (EDTA) top tube

Handling

Allow specimen to clot completely at room temperature (minimum: 30 minutes). Centrifuge and separate serum from cells ASAP.

Preferred Volume

2 mL Serum or Plasma

Minimum Volume

1 mL Serum or Plasma

Transport

Refrigerated (2-8°C)

Rejection Criteria

Serum that has been in contact with serum separator gel

Stability

Ambient (20-25°C): 1 week; Refrigerated (2-8°C): 1 month; Frozen (at or below -20°C): 3 months

Testing Information

Performed

Tue - Sat

Reported

24-48 hours

Method

Liquid Chromatography Tandem Mass Spectrometry

Reference Values

Reference Values

Methadone: Not Detected  < 30 ng/mL

Therapeutic Range

Toxic Levels

Comments

Interpret with caution as therapeutic and toxic ranges have significant overlap due to large variation in patient opioid tolerance.

CPT Codes

80358; G0480

Last Updated: Friday, June 21, 2019 01:12:08 AM


Client Services: 503-413-1234