Prenatal Panel 2 Expanded Mnemonic PREE-2 Includes ABO Group & Rh Type, Antibody Screen, CBC with Auto Diff, Syphilis Antibody Screen with Reflex, Rubella IgG Antibody, Hepatitis B Surface Antigen with Reflex Guidelines Please refer to our Syphilis LabAlert Specimen Collection Requirements Collect Whole Blood, Three FULL 4.0 mL lavender (EDTA) top tubes -AND- Serum, Two 5.0 mL gold (SST) or One 8.5 mL Red-Grey Marble top tubes Handling EDTA WB: One lavender top tube used for CBC must be at least half full & not over 24 hours old. Do not under- or over-fill tubes. The two lavender top tubes for blood typing & Ab screen must be labeled appropriately. See Blood Bank Collection Guidelines. Serum: Allow serum to clot completely at room temperature (minimum: SST - 30 minutes). Separate serum or plasma from cells within 1 hour of collection. Preferred Volume EDTA WB: 12.0 mL in three full 4.0 mL lavender top tubes Serum: 5.5 mL Minimum Volume EDTA WB: 6.0 mL in one full 4.0mL and one 2.0 mL lavender top tubes Serum: 2.5 mL (6.25 mL minimum whole blood draw) Transport EDTA WB: Refrigerated (2-8 ºC) Serum: Refrigerated (2-8 ºC) Rejection Criteria EDTA WB and Serum: Hemolyzed specimens, Contaminated with bacteria, Grossly lipemic or turbid specimens, Incorrect specimen container, Incorrectly labeled, Specimen too old EDTA WB Only: Frozen, Clotted, Tube less than half full Stability EDTA WB: Room Temperature (18-26°C): 12 hours; Refrigerated (2-8°C): 24 hours; Frozen (≤ -20°C): Unacceptable Serum: Room Temperature (18-26°C): 8 hours; Refrigerated (2-8°C): 48 hours; Frozen (≤ -20°C): 2 months Testing Information Performed Mon - Fri Reported Most tests reported within 24 hours. Refer to each test for more information. Method Refer to specific test. Reference Values Reference Values Refer to specific test. Comments Both Syphilis Screen and Hepatitis B Surface Ab will reflex to confirmatory tests for additional charge. For more information, refer to specific test. CPT Codes ABO Group, Rh Type, & Ab Screen: 86900, 86901; CBC with Auto Diff: 85025; Syphilis Screen with Reflex: 86780, 86592, 86593, 86780; Rubella IgG: 86762; Hepatitis Bs Ag with Reflex: 87340, 87341 Last Updated: Wednesday, December 4, 2019 08:15:56 PM Client Services: 503-413-1234