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PYRUVIC ACID

Mnemonic

PYRU

Synonym

Pyruvate

Specimen Collection Requirements

Patient Preparation

Patient should be fasting and at complete rest. Patient should avoid any exercise of the arm or hand before or during collection. Draw the specimen without the use of a tourniquet or within three minutes of applying the tourniquet, but before releasing the tourniquet.

Collect

Whole Blood, one green (sodium or lithium heparin) top tube

Handling

REQUIRES SPECIAL HANDLING. Immediately after blood is drawn, add exactly 1.0 mL whole blood to a chilled pyruvate collection tube containing 2.0 mL 8% (w/v) perchloric acid. Collection tubes are available from the Legacy Referral Department. Mix well for 30 seconds and place in an ice bath for 10 minutes. Centrifuge for 10 minutes at 1,500 x g. Decant supernatant into a transport tube and freeze.

Preferred Volume

2.0 mL

Minimum Volume

1.0 mL

Transport

Frozen

Rejection Criteria

If less than 1.0 mL of blood is added to the collection tube, pH of the supernatant will be too low for testing to be performed.

Stability

Ambient: Unacceptable; Refrigerated: 2 days; Frozen: 4 weeks

Testing Information

Performed

7 days/week

Reported

1-2 days

Method

Enzymatic

Referral Lab

ARUP (80310)

Reference Values

Reference Values

0.030-0.107 mmol/L (venous blood)

CPT Codes

84210

Last Updated: Wednesday, December 9, 2015 09:57:33 AM


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