RhEe ANTIGEN (RHCE) GENOTYPING Synonym Rh Genotyping E/e Antigen Specimen Collection Requirements Collect Cultured Cells (preferred): Two T-25 flasks at 80% confluent of cultured amniocytes, Amniotic fluid: 10 mL unspun fluid at 20-25°C Handling CRITICAL AMBIENT. Fill flasks with culture media to ship at 20-25°C. Backup cultures must be retained at the client's institution until testing is complete. Samples must be received within 24 hours of collection at Legacy Central Lab by noon Monday through Friday. Preferred Volume Two T-25 flasks at 80% confluent cultured amniocytes, 10.0 mL unspun amniotic fluid, 3.0 mL whole blood Minimum Volume Amniotic Fluid: 5 mL Cultured Amniocytes: Two T-25 flasks Maternal Cell Contamination Specimen: 1 mL Whole Blood (Parental Genotyping): 1 mL Transport Amniotic fluid: Room temperature. Cultured Amniocytes: CRITICAL ROOM TEMPERATURE. Must be received within 48 hours of shipment due to liability of cells. Whole Blood or Maternal Cell Contamination Specimen: Refrigerated. Stability Fetal Specimen: Ambient: 48 hours; Refrigerated: Unacceptable; Frozen: Unacceptable Whole Blood or Maternal Cell Contamination Specimen: Ambient: 72 hours; Refrigerated: 1 week; Frozen: Unacceptable Testing Information Performed Tue, Thur, Sat Reported 2-7 days Method Polymerase Chain Reaction/Fluorescent Monitoring Referral Lab ARUP (50423) Reference Values Reference Values Interpretation provided. CPT Codes 81403 Last Updated: Thursday, March 14, 2019 12:51:06 PM Client Services: 503-413-1234