This is not a diagnostic test for sickle cell trait or disease. Positive results require further studies and interpretation.
Blood samples from infants and from newborns, up to six months of age, can have up to 25% HgbF present which can cause false negatives. Sickle cell solubility testing is not performed on patients less than 6 months of age. Hemoglobin electrophoresis may be ordered as an alternate testing method.