Syphilis Antibody Screen with Reflex Mnemonic SYPH AB RFLX Synonym Syphilis Screen with Reflex, Reverse Algorithm - RPR - TP-PA - Treponemal Test - Non-treponemal Test Includes Screen: Syphilis Antibody by CIA Possible confirmation, if indicated: RPR Confirmation with Reflex to Titer and TP-PA T. pallidum Antibody by TP-PA Guidelines Please see Syphilis Testing Algorithms Test is for patients ≥30 days old, including pregnant females For patients <30 days old, see Syphilis Screen Neonatal For CSF testing, see VDRL, Cerebrospinal Fluid Specimen Collection Requirements Collect Serum, one 5.0 mL gold (SST) or 7.0 mL red top tube View image for Supply Item Number 101235 or 101192 Handling Allow serum tubes to clot completely at room temperature (minimum - SST: 30 minutes, red top tubes: 60 minutes). Centrifuge and separate serum from cells within 24 hours of collection. Preferred Volume 3.0 mL Serum Minimum Volume 1.5 mL Serum (4.5 mL minimum whole blood) Transport Refrigerated (2-8 ºC) Rejection Criteria Grossly hemolyzed or plasma specimens Grossly lipemic or turbid specimens CSF Stability AFTER SEPARATION FROM CELLS: Room Temperature (18-26°C): 6 days; Refrigerated (2-8°C): 6 days; Frozen (≤ -20°C): 1 month Testing Information Performed 7 days/week Reported 24-48 hours Method Screen: Chemiluminescent Immunoassay Possible confirmation, if indicated: RPR Cardiolipin Card Flocculation Assay T. Pallidum Particle Agglutination Reference Values Reference Values Non-Reactive Please see Syphilis Testing Algorithms Comments Reactive Syphilis Antibody by CIA results are automatically reflexed to RPR. Reactive RPR results will be titered. Non-reactive RPR results will be reflexed to T. Pallidum Antibody by TP-PA for confirmation. CPT Codes Syphilis Ab: 86780; RPR: 86592; RPR Titer: 86593; TP-PA: 86780 Last Updated: Monday, February 15, 2021 06:55:16 PM Client Services: 503-413-1234