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TYPE AND CROSSMATCH

Mnemonic

RC PRD

Synonym

Compatibility Testing - Crossmatch

Includes

ABO and Rh Type, Antibody Screen (crossmatch techniques vary depending upon results of antibody screen). Antibody Identification and Antiglobulin Crossmatch is performed on patients with a positive antibody screen or a history of previous red cell antibodies. Selected Antigen Testing of patient specimen may be required in patients with red cell antibodies.

Guidelines

Preparation for transfusion of blood. Test is indicated when transfusion is likely within 24 hours; during a surgical procedure; for pre-surgical patients.

Specimen Collection Requirements

Patient Preparation

Patient should have patient identification wristband attached at the time of phlebotomy. Correct identification of the patient is critical.

Collect

Adult: Two full 4.0 mL lavender (EDTA) top tubes Neonates <4 months: Two full lavender top microtainer tubes Pediatrics ≥4 months: 3-6 full lavender top microtainer tubes

Handling

Tubes must be labeled appropriately. See Blood Bank Specimen Collection Guidelines.

Preferred Volume

Two full 4.0 mL lavender (EDTA) top tubes

Minimum Volume

Two lavender (EDTA) top microtainer tubes (Whole Blood 1.0 mL minimum draw). Neonates <4 months: One lavender (EDTA) top microtainer tube (whole Blood 0.5 mL minimum draw)

Transport

Ambient or refrigerated

Rejection Criteria

Gross hemolysis, incorrect specimen container, incorrectly labeled or frozen

Stability

Ambient: 24 hours; Refrigerated: 3 days; Frozen: Unacceptable

Testing Information

Performed

7 days/week

Reported

Routine Testing: 2-24 hours, additional time should be allowed for patients known to be sensitized to red cell antigens. Time is needed when unanticipated problems arise.

Method

Electronic (computer) crossmatch. For patients who are sensitized to red cell antigens, an antiglobulin crossmatch is required.

Reference Values

Comments

Storage: Crossmatched blood will be held for a patient for 3 days. After that time it will be released without notification to the physician. It is the physician's responsibility to place further orders.

Expiration: Patient sample is used for crossmatch or antibody screen only if it is less than 3 days old. When it is known that the patient has not been recently transfused or pregnant, this time can be extended to 28 days, for pre-surgical patients.

Limitations: Does not assure normal red cell survival. Will not detect all antibodies or incompatibilities. Does not prevent all transfusion reactions.

CPT Codes

86900, 86901, 86850, 86923

Last Updated: Thursday, March 14, 2019 12:51:13 PM


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