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Vitamin D, 25-Hydroxy

 

Mnemonic

VITD 25

Synonym

Vitamin D - 25-Hydroxyvitamin D2 - 25-Hydroxyvitamin D3 - Calciferol, 25-Hydroxycalciferol - Cholecalciferol - Ergocalciferol

Guidelines

Preferred test to diagnose vitamin D insufficiency and monitor response to therapy. Testing is recommended only for patients at risk for vitamin D insufficiency.

Specimen Collection Requirements

Collect

Serum, one 5.0 mL gold (SST) or 7.0 mL red top tube

Handling

Allow serum to clot completely at room temperature (minimum – SST: 30 minutes; red top tubes: 60 minutes). Centrifuge and separate serum from cells within 23 hours of collection.

Preferred Volume

1.0 mL Serum

Minimum Volume

0.5 mL Serum (1.2 mL minimum whole blood draw)

Transport

Refrigerated (2-8°C)

Rejection Criteria

Plasma, grossly hemolyzed samples, grossly lipemic samples, samples containing particulate matter or exhibiting obvious microbial contamination, and samples frozen and thawed more than 4 times.

Stability

WHOLE BLOOD (UNSPUN): Room Temperature (18-26°C): 23 hours; Refrigerated (2-8°C): 23 hours; Frozen (≤-20°C): unacceptable

 

SERUM (SEPARATED FROM CELLS, POST COLLECTION): Room Temperature (18-26°C): 23 hours; Refrigerated (2-8°C): 5 days; Frozen (≤-20°C): 8 weeks

Do not exceed 4 freeze-thaw cycles.

Testing Information

Performed

Mon-Fri

Reported

1-3 days

Method

Diasorin Liaison XL Chemiluminescent Immunoassay

Reference Values

Reference Values

25-Hydroxyvitamin D (ng/mL)

Interpretation

0.0 - 14.9

Deficiency

15.0 – 29.9

Insufficiency

30.0 – 99.9

Optimal

>99.9

Possible Toxicity

CPT Codes

82306

Last Updated: Wednesday, January 22, 2020 03:39:58 PM


Client Services: 503-413-1234